Optimization of Alkaline Single Cell Gel Electropohoresis (Comet Assay) Protocol for Cells from Oral Cavity
نویسندگان
چکیده
منابع مشابه
Improvement of single-cell gel electrophoresis (SCGE) alkaline comet assay
Although the single-cell gel electrophoresis (SCGE) alkaline comet assay can detect DNA damage quickly and with high sensitivity, it does not work well for cryopreserved sperm of some marine teleosts. Using large yellow croaker Pseudosciaena crocea sperm that were cryopreserved in increasing concentrations (5 to 30%) of DMSO, we made modifications to the classic SCGE method that included using ...
متن کاملDRAFT OECD GUIDELINE FOR THE TESTING OF CHEMICALS Rodent alkaline single cell gel electrophoresis (Comet) assay INTRODUCTION
1. OECD Test Guidelines (TGs) are available for a wide range of in vitro genotoxicity assays that are able to detect DNA damage, gene mutation and/or chromosomal aberrations. There are TGs for in vivo endpoints (i.e. chromosomal aberrations, gene mutations and DNA repair as unscheduled DNA synthesis); however, these do not directly measure DNA damage. The alkaline single cell gel electrophoresi...
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To monitor genotoxicity in small bodies of water (e.g., creeks, ponds, and drainage ditches) we examined tadpole erythrocytes of two species: Rana clamitans and Rana pipiens,using the alkaline single cell gel DNA electrophoresis (SCG) or "comet" assay. This approach involves detection, under alkaline conditions, of cell DNA fragments which on electrophoresis migrate from the nuclear core, resul...
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Pesticides are broadly used for pest control in agriculture despite possible negative impacts they may pose to the environment. Thus, we examined the DNA damage caused by five herbicides commonly used in southern Ontario (Canada). Erythrocytes from Rana catesbeiana (bullfrog) tadpoles were evaluated for DNA damage following exposure to selected herbicides, using the alkaline single-cell gel DNA...
متن کاملComparison of sensitivity to methyl methanesulphonate among tadpole developmental stages using the alkaline single-cell gel electrophoresis (comet) assay.
In a previous study, we demonstrated that tadpoles are suitable organisms for monitoring small bodies of water (e.g., creeks, ponds, and drainage ditches) for genotoxicity using the alkaline single-cell gel DNA electrophoresis (SCG) or "comet" assay [Ralph and Petras, 1997]. This approach involves detection, under alkaline conditions, of cell DNA fragments which on electrophoresis migrate from ...
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ژورنال
عنوان ژورنال: Journal of Advances in Medicine and Medical Research
سال: 2018
ISSN: 2456-8899
DOI: 10.9734/jammr/2018/38227